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Squamous cell carcinoma of the anal canal showing a markedly stronger nuclear NFIX staining of tumor than of stromal cells

Staining Pattern in Normal Tissues

Manual protocol

Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply HMV329 at a dilution of 1:200 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Brain
CerebrumStrong nuclear NFIX staining of glial cells. Weak or absent staining in neurons.
CerebellumDistinct nuclear NFIX staining of glial cells, moderate staining of granular cells, and lack of staining in Purkinje cells.
Endocrine tissues
ThyroidDistinct nuclear NFIX staining of follicular cells.
ParathyroidModerate nuclear NFIX staining of all epithelial cells.
Adrenal glandMedulla with distinct nuclear NFIX staining of few interspersed stromal (sustentacular cells? endothelial?) cells while adrenal medullary cells remain NFIX negative. Weak to moderate staining of adrenocortical cells.
Pituitary glandDistinct nuclear NFIX staining of all pituicytes in the neurohypophysis while only few (epithelial??) cells of the adenohypophysis show a distinct nuclear NFIX staining.
Respiratory system
Respiratory epitheliumDistinct nuclear NFIX staining of the cells of the respiratory epithelium, but the staining intensity decreases from the basal layer towards the more superficial cell layers. Strong nuclear NFIX staining of bronchial gland cells.
LungStrong nuclear NFIX staining of all cell types (except macrophages).
Gastrointestinal tract
Salivary glandsStrong nuclear NFIX staining of epithelial cells from glands and excretory ducts.
EsophagusDistinct nuclear NFIX staining of all cells. Significant decrease of NFIX staining of squamous epithelium from the basal/suprabasal to the superficial cell layers.
StomachModerate to strong nuclear NFIX staining of most epitheliall cells but few glandular cells do only show faint or even absent staining.
DuodenumNuclear NFIX staining is strong in Brunner glands, moderate in crypt bases and weak/faint or even absent in the surface epithelium.
Small intestineNuclear NFIX staining is moderate in crypt bases and weak/faint or even absent in the surface epithelium.
AppendixModerate to strong nuclear NFIX staining of epithelial cells.
ColonWeak to moderate to strong nuclear NFIX staining of epithelial cells. Slight but significant decrease of NFIX staining intensity from the crypt base to the superficial epithelial cell layers.
RectumWeak to moderate to strong nuclear NFIX staining of epithelial cells. Slight but significant decrease of NFIX staining intensity from the crypt base to the superficial epithelial cell layers.
Liver, Gallbladder, Pancreas
LiverNuclear NFIX staining is largely absent in hepatocytes. NFIX staining is seen in stromal cells.
GallbladderNuclear NFIX staining is weak to moderate in epithelial cells but stronger in stromal cells.
PancreasStrong nuclear NFIX staining of epithelial cells of excretory ducts and of intercalated ducts. NFIX staining is distinctively weaker and sometimes absent in acinar cells.
Genitourinary
KidneyNuclear NFIX staining is generally low in all epithelial cell types. Staining is variable ranging from absent to faint/weak.
UrotheliumDistinct nuclear NFIX staining of urothelial cells but there is a significant decrease of NFIX staining intensity from the basal to the superficial cell layers. Umbrella cells are largely NFIX negative.
Male tissues
ProstateStrong nuclear NFIX staining of stromal and of all epithelial cells.
Seminal VesiclesStrong nuclear NFIX staining of stromal and basal epithelial cells. NFIX staining is weak or absent in most luminal epithelial cells.
TestisDistinct nuclear NFIX staining of intertubular cells (including Leydig cells). Complete absence of NFIX staining in tubular cells (Sertoli cells, germ cells).
EpididymisStrong nuclear NFIX staining of epithelial cells in the cauda. In the caput, the NFIX staining is only faint or absent in chief cells but strong in basal cells.
Female tissues
BreastStrong nuclear NFIX staining of a large subset of basal and luminal epithelial cells.
Uterus, myometriumStrong nuclear NFIX staining of smooth muscle cells.
Uterus, ectocervixDistinct nuclear NFIX staining of squamous epithelial cells although there is a tendency towards a decreased staining intensity in the superficial cell layers.
Uterus, endocervixModerate nuclear NFIX staining of glandular cells. Strong nuclear NFIX staining of stromal cells.
Uterus, endometriumStrong nuclear NFIX staining of stromal cells while endometrium cells remain NFIX negative.
Fallopian tubeStrong nuclear NFIX staining of all cells.
OvaryStrong nuclear NFIX staining of stromal cells. Corpus luteum cells are NFIX negative.
Placenta earlyStrong nuclear NFIX staining of stromal cells while trophoblast cells remain negative.
Placenta matureStrong nuclear NFIX staining of stromal cells while trophoblast cells remain negative.
AmnionNegative
ChorionNegative
Skin
EpidermisStrong nuclear NFIX staining of all squamous epithelial cells.
Sebaceous glandsWeak to moderate nuclear NFIX staining of sebaceous gland cells. The staining intensity decreases towards the centre of the glands.
Muscle, connective & soft tissues
Heart muscleStrong nuclear NFIX staining of all muscle cells.
Skeletal muscleStrong nuclear NFIX staining of all muscle cells.
Smooth muscleStrong nuclear NFIX staining of all muscle cells.
Vessel wallsStrong nuclear NFIX staining of endothelial and muscle cells.
FatStrong nuclear NFIX staining of fat cells.
StromaVariable (often strong) nuclear NFIX staining of various types of stroma cells.
EndotheliumStrong nuclear NFIX staining of all endothelial cells.
Bone marrow & lymphoid tissues
Bone marrowStrong nuclear NFIX staining of a rather small subset of cells (often arranged in groups).
Lymph nodeDistinct nuclear NFIX staining of stromal and endothelial cells. Lymphocytes are NFIX negative.
SpleenDistinct nuclear NFIX staining of stromal and endothelial cells. Lymphocytes are NFIX negative.
ThymusLymphocytes are NFIX negative. Distinct nuclear NFIX staining of epithelial cells. Maturing squamous epithelial cells of corpuscles of Hassall’s do only stain weakly or remain negative.
TonsilDistinct nuclear NFIX staining of squamous epithelial cells although there is a tendency towards a decreased staining intensity in the superficial cell layers. Lymphocytes are largely negative.
Remarks

The majority of cell types show a variable, often strong nuclear NFIX staining.

NFIX

(HMV329)

NFIX is a master transcription factor interacting with many other transcription factors and chromatin.

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NFIX (HMV329)
€295.00

Details

Type
Recombinant Rabbit monoclonal / IgG
Clone
HMV329
Reactivity
Human

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Biology behind

Nuclear factor 1 X-type (NFIX) is a transcription factor protein which is coded by the NFIX gene on chromosome 19p13.13. Besides NFIA, NFIB, NFIC, NFIX is one out of four closely related members of the nuclear factor I (NFI) family of transcription factors. The family members share a particularly high rate of interactions with other transcription factors. They are therefore thought to not only directly regulate the expression of genes but also believed to modulate the function of other transcription factors. NFIX is known to play a role in muscle and central nervous system embryonic development. For example, NFIX has been shown to control the timing of neural differentiation promoting the ongoing growth of the hippocampus and proper memory function. The role of NFIX in other tissues is less intensively studied. Various types of NFIX alterations have been found in tumors. Mechanisms leading to both increased and reduced expression have been found to promote pro-tumorigenic functions, such as increased cell proliferation and migration and disturbed differentiation.

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