Staining Pattern in Normal Tissues
Manual protocol
Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply HMV309 at a dilution of 1:200 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Brain | Cerebrum | Negative | |
Cerebellum | A weak TRIM72 staining of fibres in the molecular layer may occur. | ||
Endocrine tissues | Thyroid | Negative | |
Parathyroid | Negative | ||
Adrenal gland | Negative | ||
Pituitary gland | Negative | ||
Respiratory system | Respiratory epithelium | Negative | |
Lung | Negative | ||
Gastrointestinal tract | Salivary glands | Weak to moderate, primarily cytoplasmic TRIM72 staining of a subset of excretory duct epithelial cells. | |
Esophagus | Negative | ||
Stomach | Negative | ||
Duodenum | Negative | ||
Small intestine | Negative | ||
Appendix | Negative | ||
Colon | Negative | ||
Rectum | Negative | ||
Liver, Gallbladder, Pancreas | Liver | Negative | |
Gallbladder | Negative | ||
Pancreas | Negative | ||
Genitourinary | Kidney | TRIM72 staining of arterioles and small arteries can occur. | |
Urothelium | Negative | ||
Male tissues | Prostate | Negative | |
Seminal Vesicles | Negative | ||
Testis | Negative | ||
Epididymis | Negative | ||
Female tissues | Breast | Negative | |
Uterus, myometrium | Negative | ||
Uterus, ectocervix | Negative | ||
Uterus, endocervix | Negative | ||
Uterus, endometrium | Negative | ||
Fallopian tube | Negative | ||
Ovary | Negative | ||
Placenta early | Negative | ||
Placenta mature | Negative | ||
Amnion | Negative | ||
Chorion | Negative | ||
Skin | Epidermis | Negative | |
Sebaceous glands | Negative | ||
Muscle, connective & soft tissues | Heart muscle | Negative | |
Skeletal muscle | Strong membranous and cytoplasmic TRIM72 staining of skeletal muscle cells. | ||
Smooth muscle | TRIM72 staining of the walls of vessels can be found in the smooth muscular wall of various organs, especially in the GIT. | ||
Vessel walls | A weak to moderate, predominantly cytoplasmic TRIM72 staining of muscle cells of the walls of small vessels can occur. These include muscular small arteries, arterioles, and perhaps also venous vessels. | ||
Fat | Negative | ||
Stroma | Negative | ||
Endothelium | Negative | ||
Bone marrow & lymphoid tissues | Bone marrow | Negative | |
Lymph node | Negative | ||
Spleen | Negative | ||
Thymus | Distinct TRIM72 staining of a small subset of epithelial cells some of which are associated with corpuscles of Hassall‘s. | ||
Tonsil | Negative | ||
Remarks | A weak to moderate cytoplasmic TRIM72 staining of muscle cells of the walls of small vessels can occur in various organs. A limitation of this phenomenon to specific organs/tissues cannot be excluded. |
Details
More product details
More product details
Biology behind
Tripartite motif protein 72 (TRIM72) is one out of a family of 80 tripartite motif proteins. It is coded by the TRIM72 gene on chromosome 16p11.2 and consists of four domains: RING domain, B-box domain, coiled-coil domain, and SPRY domain. TRIM72 is predominantly expressed in skeletal muscle. Muscle cell membrane repair is believed to represent its main function but TRIM72 also has roles in calcium homeostasis, muscle contraction, vesicle trafficking, anti-inflammation, reduction of oxidative stress, and regulation of systemic insulin response.
Protocol Recommendations
Protocol Recommendations
Potential Research Applications
Potential Research Applications
Evidence For Antibody Specificity In I H C
Evidence For Antibody Specificity In I H C