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Skeletal muscle cells with a strong membranous and cytoplasmic TRIM72 staining.

Staining Pattern in Normal Tissues

Manual protocol

Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply HMV309 at a dilution of 1:200 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Brain
CerebrumNegative
CerebellumA weak TRIM72 staining of fibres in the molecular layer may occur.
Endocrine tissues
ThyroidNegative
ParathyroidNegative
Adrenal glandNegative
Pituitary glandNegative
Respiratory system
Respiratory epitheliumNegative
LungNegative
Gastrointestinal tract
Salivary glandsWeak to moderate, primarily cytoplasmic TRIM72 staining of a subset of excretory duct epithelial cells.
EsophagusNegative
StomachNegative
DuodenumNegative
Small intestineNegative
AppendixNegative
ColonNegative
RectumNegative
Liver, Gallbladder, Pancreas
LiverNegative
GallbladderNegative
PancreasNegative
Genitourinary
KidneyTRIM72 staining of arterioles and small arteries can occur.
UrotheliumNegative
Male tissues
ProstateNegative
Seminal VesiclesNegative
TestisNegative
EpididymisNegative
Female tissues
BreastNegative
Uterus, myometriumNegative
Uterus, ectocervixNegative
Uterus, endocervixNegative
Uterus, endometriumNegative
Fallopian tubeNegative
OvaryNegative
Placenta earlyNegative
Placenta matureNegative
AmnionNegative
ChorionNegative
Skin
EpidermisNegative
Sebaceous glandsNegative
Muscle, connective & soft tissues
Heart muscleNegative
Skeletal muscleStrong membranous and cytoplasmic TRIM72 staining of skeletal muscle cells.
Smooth muscleTRIM72 staining of the walls of vessels can be found in the smooth muscular wall of various organs, especially in the GIT.
Vessel wallsA weak to moderate, predominantly cytoplasmic TRIM72 staining of muscle cells of the walls of small vessels can occur. These include muscular small arteries, arterioles, and perhaps also venous vessels.
FatNegative
StromaNegative
EndotheliumNegative
Bone marrow & lymphoid tissues
Bone marrowNegative
Lymph nodeNegative
SpleenNegative
ThymusDistinct TRIM72 staining of a small subset of epithelial cells some of which are associated with corpuscles of Hassall‘s.
TonsilNegative
Remarks

A weak to moderate cytoplasmic TRIM72 staining of muscle cells of the walls of small vessels can occur in various organs. A limitation of this phenomenon to specific organs/tissues cannot be excluded.

TRIM72

(HMV313)

TRIM72 is a marker for skeletal muscle cells.

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TRIM72 (HMV313)
€295.00

Details

Type
Recombinant Rabbit monoclonal / IgG
Clone
HMV313
Reactivity
Human

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Biology behind

Tripartite motif protein 72 (TRIM72) is one out of a family of 80 tripartite motif proteins. It is coded by the TRIM72 gene on chromosome 16p11.2 and consists of four domains: RING domain, B-box domain, coiled-coil domain, and SPRY domain. TRIM72 is predominantly expressed in skeletal muscle. Muscle cell membrane repair is believed to represent its main function but TRIM72 also has roles in calcium homeostasis, muscle contraction, vesicle trafficking, anti-inflammation, reduction of oxidative stress, and regulation of systemic insulin response.

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Potential Research Applications

Evidence For Antibody Specificity In I H C