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Skeletal muscle cells with a strong membranous and cytoplasmic TRIM72 staining.

Staining Pattern in Normal Tissues

Manual protocol

Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply HMV309 at a dilution of 1:200 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

BrainCerebrum, grey Negative
Cerebrum, white Negative
Cerebellum, cortex Negative
Cerebellum, white Negative
Ganglion Negative
Ependyma Negative
Eye, retina Negative
Endocrine TissuesThyroid Negative
Parathyroid gland Negative
Adrenal gland Negative
Pituitary gland, anterior lobe Negative
Pituitary gland, posterior lobe Negative
Respiratory systemLung bronchi Negative
Lung, bronchial glands Negative
Nose, paranasal sinus Negative
Lung, parenchyma Negative
Proximal digestive tractLip Negative
Oral cavity Negative
Tonsil, surface Negative
Esophagus, mucosa Negative
Lip, small salivary gland Negative
Sublingual gland Negative
Parotid gland Negative
Submandibullary gland Negative
Gastronintestinal tractStomach, antrum Negative
Stomach, fundus and corpus Negative
Small intestine, duodenum Negative
Duodenum, Brunner gland Negative
Small intestine, ileum Negative
Appendix Negative
Colon descendens Negative
Rectum Negative
Anal canal, transition epithelium Negative
Liver, Gallbladder, PancreasLiver Negative
Gallbladder Negative
Pancreas Negative
Kidney, urinary bladderKidney, cortex Negative
Kidney, medulla Negative
Urinary bladder, urothelium Negative
Kidney pelvis, mucosa Negative
Male tissuesProstate Negative
Seminal vesicle Negative
Epididymis caput Negative
Epididymis cauda Negative
Testis Negative
Female TissuesBreast, glands Negative
Ectocervix Negative
Endocervix Negative
Endometrium, proliferation Negative
Endometrium, secretion Negative
Uterus, myometrium Negative
Fallopian tube Negative
Ovary, stroma Negative
Ovary, follicular cyst Negative
Ovary, corpus luteum Negative
Amnion Negative
Chorion Negative
Amnion/Chorion Negative
Placenta, early, decidua Negative
Placenta, first trimenon Negative
Placenta, mature Negative
Muscle, connective & soft tissueAorta, intima Negative
Skeletal muscle Strong membranous and cytoplasmic TRIM72 staining of skeletal muscle cells.
Aorta, media Negative
Skeletal muscle, tongue Negative
Heart, left ventricle Negative
Kidney pelvis, muscular wall Negative
Urinary bladder, muscular wall Negative
Esophagus, muscular wall Negative
Stomach, muscular wall Negative
Ileum, muscular wall Negative
Appendix, muscular wall Negative
Colon descendens, muscular wall Negative
Penis, glans, corpus spongiosum Negative
Fat, white Negative
SkinSkin, surface Negative
Skin (hairs, sebaceous glands) Negative
Anal canal, skin Negative
Scrotum Negative
Bone Marrow & lymphoid tissuesBone marrow Negative
Thymus Distinct TRIM72 staining of a small subset of epithelial cells some of which are associated with corpuscles of Hassall‘s.
Spleen Negative
Lymph node Negative
Tonsil, deep Negative

TRIM72

(HMV313)

TRIM72 is a marker for skeletal muscle cells.

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From €295.00
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TRIM72 (HMV313)—
€295.00

Details

Type
Recombinant Rabbit monoclonal / IgG
Clone
HMV313
Reactivity
Human

More product details

Biology behind

Tripartite motif protein 72 (TRIM72) is one out of a family of 80 tripartite motif proteins. It is coded by the TRIM72 gene on chromosome 16p11.2 and consists of four domains: RING domain, B-box domain, coiled-coil domain, and SPRY domain. TRIM72 is predominantly expressed in skeletal muscle. Muscle cell membrane repair is believed to represent its main function but TRIM72 also has roles in calcium homeostasis, muscle contraction, vesicle trafficking, anti-inflammation, reduction of oxidative stress, and regulation of systemic insulin response.

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