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Papillary thyroid cancer with strong nuclear TRIM28 immunostaining of tumor cells.

Staining Pattern in Normal Tissues

Manual protocol

Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply HMV335 at a dilution of 1:200 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Brain
CerebrumStrong nuclear TRIM28 staining in all cells.
CerebellumStrong nuclear TRIM28 staining in all cells.
Endocrine tissues
ThyroidStrong nuclear TRIM28 staining in all cells.
ParathyroidStrong nuclear TRIM28 staining in all cells.
Adrenal glandStrong nuclear TRIM28 staining in all cells.
Pituitary glandStrong nuclear TRIM28 staining in all cells.
Respiratory system
Respiratory epitheliumStrong nuclear TRIM28 staining in all cells.
LungStrong nuclear TRIM28 staining in all cells.
Gastrointestinal tract
Salivary glandsStrong nuclear TRIM28 staining in all cells.
EsophagusSlight decrease of the staining intensity from basal to superficial cell layers  of squamous eithelium.
StomachStrong nuclear TRIM28 staining in all cells.
DuodenumStrong nuclear TRIM28 staining in all cells.
Small intestineStrong nuclear TRIM28 staining in all cells.
AppendixStrong nuclear TRIM28 staining in all cells.
ColonSlight decrease of the nuclear TRIM28 staining intensity from crypt base to the surface epithelium.
RectumSlight decrease of the nuclear TRIM28 staining intensity from crypt base to the surface epithelium.
Liver, Gallbladder, Pancreas
LiverThe intensity of nuclear TRIM28 staining is less intense in hepatocytes than in other cell types (in some saples even completely missing).
GallbladderStrong nuclear TRIM28 staining in all cells.
PancreasStrong nuclear TRIM28 staining in all cells.
Genitourinary
KidneyStrong nuclear TRIM28 staining in all cells.
UrotheliumStrong nuclear TRIM28 staining in all cells.
Male tissues
ProstateStrong nuclear TRIM28 staining in all cells.
Seminal VesiclesStrong nuclear TRIM28 staining in all cells.
TestisStrong nuclear TRIM28 staining in all cells. Exception: Spermatids are TRIM28 negative.
EpididymisStrong nuclear TRIM28 staining in all cells.
Female tissues
BreastStrong nuclear TRIM28 staining in all cells.
Uterus, myometriumStrong nuclear TRIM28 staining in all cells.
Uterus, ectocervixSlight decrease of the staining intensity from basal to superficial cell layers  of squamous eithelium.
Uterus, endocervixStrong nuclear TRIM28 staining in all cells.
Uterus, endometriumStrong nuclear TRIM28 staining in all cells.
Fallopian tubeStrong nuclear TRIM28 staining in all cells.
OvaryStrong nuclear TRIM28 staining in all cells.
Placenta earlyStrong nuclear TRIM28 staining in all cells.
Placenta matureStrong nuclear TRIM28 staining in all cells.
AmnionStrong nuclear TRIM28 staining in all cells.
ChorionStrong nuclear TRIM28 staining in all cells.
Skin
EpidermisStrong nuclear TRIM28 staining in all cells.
Sebaceous glandsStrong nuclear TRIM28 staining in all cells.
Muscle, connective & soft tissues
Heart muscleStrong nuclear TRIM28 staining in all cells.
Skeletal muscleStrong nuclear TRIM28 staining in all cells.
Smooth muscleStrong nuclear TRIM28 staining in all cells.
Vessel wallsStrong nuclear TRIM28 staining in all cells.
FatStrong nuclear TRIM28 staining in all cells.
StromaStrong nuclear TRIM28 staining in all cells.
EndotheliumStrong nuclear TRIM28 staining in all cells.
Bone marrow & lymphoid tissues
Bone marrowStrong nuclear TRIM28 staining in all cells.
Lymph nodeStrong nuclear TRIM28 staining in all cells.
SpleenStrong nuclear TRIM28 staining in all cells.
ThymusStrong nuclear TRIM28 staining in all cells.
TonsilStrong nuclear TRIM28 staining in all cells.
Remarks

KAP1 / TRIM28

(HMV335)

KAP1 / TRIM28 is a multifunctional regulatory nuclear protein.

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KAP1 / TRIM28 (HMV335)
€295.00

Details

Type
Recombinant Rabbit monoclonal / IgG
Clone
HMV335
Reactivity
Human

More product details

Biology behind

Tripartite motif-containing 28 (TRIM28), also termed KAP1 (KRAB-associated protein-1), or transcriptional mediator 1b (TIF1b) is a regulatory protein coded by the TRIM28 gene at chromosome 19q13.43. The protein plays a role in transcription regulation by interaction with the Krüppel-associated box repression domain found in many transcription factors and binding to specific chromatin regions. In addition to regulating gene transcription, KAP1 has a variety of regulatory intracellular functions, such as response to DNA damage, maintaining stem cell pluripotency, cellular differentiation and proliferation, viral suppression, and apoptosis. KAP1 is ubiquitously expressed, and its function depends on posttranscriptional modification.

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Potential Research Applications

Evidence For Antibody Specificity In I H C