Normal Tissues

Comparison mode

NFIX

Brain

Cerebrum, grey matter

Cerebrum, grey matter – Strong nuclear NFIX staining of glial cells. Weak or absent staining in neurons

Cerebrum, white matter

Cerebrum, white matter – Strong nuclear NFIX staining of glial cells

Cerebellum, cortex (molecular layer, Purkinje cell layer, granule cell layer)

Cerebellum, cortex (molecular layer, Purkinje cell layer, granule cell layer) – Weak nuclear NFIX staining of glial and granular cells. Lack of staining NFIX in Purkinje cell

Cerebellum (white matter)

Cerebellum (white matter) – Weak to moderate nuclear NFIX staining of glial cells

Endocrine Tissues

Adrenal gland (Cortex)

Adrenal gland (Cortex) – Weak to moderate nuclear NFIX staining staining of adrenocortical cells

Adrenal gland (Medulla)

Adrenal gland (Medulla) – Distinct nuclear NFIX staining of few interspersed stromal (sustentacular cells_ endothelial_) cells while medullary cells remain negative

Thyroid gland

Thyroid gland – Strong nuclear MRE11 staining of follicular cells

Parathyroid gland

Parathyroid gland – Moderate nuclear NFIX staining of all epithelial cells

Pituitary gland, anterior lobe

Pituitary gland, anterior lobe – Only few cells show a moderate to strong nuclear NFIX staining

Pituitary gland, posterior lobe

Pituitary gland, posterior lobe – Strong nuclear NFIX staining of all pituicytes

Respiratory system

Lung

Lung – Strong nuclear NFIX staining of all cell types (except macrophages- these remain NFIX negative)

Proximal digestive tract

Duodenum, mucosa

Duodenum, mucosa – Nuclear NFIX staining is moderate in crypt bases and weak-faint or even absent in the surface epithelium

Ileum, mucosa

Ileum, mucosa – Nuclear NFIX staining is moderate in crypt bases and weak-faint or even absent in the surface epithelium

Tonsil, surface epithelium

Tonsil, surface epithelium – Significant decrease of nuclear NFIX staining of squamous epithelium from the basal-suprabasal to the superficial cell layers

Parotid gland

Gastronintestinal tract

Stomach, antrum

Stomach, antrum – Moderate to strong nuclear NFIX staining of most epithelial cells but few glandular cells do only show faint or even absent NFIX staining

Duodenum, Brunner gland

Duodenum, Brunner gland – Distinct nuclear MRE11 staining of all epithelial cells, including Brunner glands

Liver, Gallbladder, Pancreas

Liver

Liver – Nuclear NFIX staining is largely absent in hepatocytes. NFIX staining is only seen in stromal cells

Pancreas

Pancreas – Strong nuclear NFIX staining of epithelial cells of excretory ducts and of intercalated ducts. NFIX staining is markedly weaker in acinar cells

Pancreas

Pancreas – Strong nuclear NFIX staining of stromal and intercalated duct cells. NFIX staining is faint or absent in acinar cells

Kidney, urinary bladder

Kidney, cortex

Kidney, cortex – Nuclear NFIX staining is generally low in all cell types and ranges from absent to faint-weak

Kidney, medulla

Kidney, medulla – Nuclear NFIX staining is generally low in all cell types and ranges from absent to faint-weak

Kidney, pelvis, urothelium

Kidney, pelvis, urothelium – Distinct nuclear NFIX staining of urothelial cells although there is a significant decrease of NFIX staining intensity from the basal to the superficial cell layers. Umbrella cells are largely NFIX negative

Urinary bladder, urothelium

Urinary bladder, urothelium – Distinct nuclear NFIX staining of urothelial cells although there is a significant decrease of NFIX staining intensity from the basal to the superficial cell layers. Umbrella cells are largely NFIX negative

Male tissues

Epididymis (Caput and cauda)

Epididymis (Caput and cauda) – Strong nuclear NFIX staining of epithelial cells in the cauda. In the caput, the NFIX staining is only faint in chief cells but strong in basal cells

Epididymis (Cauda)

Epididymis (Cauda) – Strong nuclear NFIX staining of epithelial cells

Prostate

Prostate – Strong nuclear NFIX staining of stromal and all epithelial cells

Seminal vesicle

Seminal vesicle – Strong nuclear NFIX staining of stromal and basal epithelial cells. NFIX staining is weak or absent in most luminal epithelial cells

Testis

Testis – Distinct nuclear NFIX staining of intertubular cells (including Leydig cells). Complete absence of NFIX staining in tubular cells (Sertoli cells, germ cells)

Female Tissues

Breast

Breast – Strong nuclear NFIX staining of a large subset of basal and luminal epithelial cells

Uterus, endocervix

Uterus, endocervix – Moderate nuclear NFIX staining of glandular cells. Strong nuclear NFIX staining of stromal cells

Uterus, endometrium (proliferation)

Uterus, endometrium (proliferation) – Strong nuclear NFIX staining of stromal cells while endometrium cells remain NFIX negative

Uterus, endometrium (pregnancy)

Uterus, endometrium (pregnancy).jpeg

Uterus, endometrium (secretion)

Uterus, endometrium (secretion) – Strong nuclear NFIX staining of stromal cells while endometrium cells remain NFIX negative

Uterus, myometrium

Uterus, myometrium – Distinct nuclear staining of smooth muscle cells of the myometrium

Fallopian tube, mucosa

Fallopian tube, mucosa – Strong nuclear NFIX staining of all cells

Ovary, stroma

Ovary, stroma – Strong nuclear NFIX staining of stromal cells

Ovary, corpus luteum

Ovary, corpus luteum – Strong nuclear NFIX staining of stromal cells. Corpus luteum cells are NFIX negative

Placenta, early

Placenta, early – Strong nuclear MRE11 staining of stromal cells while trophoblast cells remain negative

Placenta (chorion)

Placenta (amnion)

Placenta, mature

Placenta, mature – Strong nuclear NFIX staining of stromal cells while trophoblast cells remain negative

Muscle, connective & soft tissue

Skeletal muscle

Skeletal muscle – Distinct nuclear staining of skeletal muscle cells and of other cell types

Aorta, media

Heart muscle

Heart muscle – Distinct nuclear staining of heart muscle cells and of other cell types

Urinary bladder, muscular wall

Urinary bladder, muscular wall – Distinct nuclear staining of smooth muscle cells and of other cell types

Stomach, muscular

Stomach, muscular – Distinct nuclear staining of smooth muscle cells and of other cell types

Appendix, muscular wall

Appendix, muscular wall – Distinct nuclear staining of smooth muscle cells and of other cell types

Colon descendens, mucosa

Colon descendens, mucosa – Moderate nuclear NFIX staining of epithelial cells

Colon descendens, muscular wall

Colon descendens, muscular wall – Distinct nuclear staining of smooth muscle cells and of other cell types

Skin

Skin – Strong nuclear NFIX staining of squamous epithelial cells

Skin, hairfollicel and sebaceous glands

Skin, hairfollicel and sebaceous glands – Weak to moderate nuclear NFIX staining of sebaceous gland cells. The staining intensity decreases towards the centre of the glands

Bone Marrow & lymphoid tissues

Bone marrow

Bone marrow – Strong nuclear MRE11 staining of a rather small subset of cells (often arranged in groups)

Thymus

Thymus – Lymphocytes are NFIX negative. Distinct nuclear NFIX staining of epithelial cells. Maturing squamous epithelial cells of corpuscles of Hassall’s stain weakly or remain negative

Thymus

Thymus – Lymphocytes are NFIX negative. Distinct nuclear NFIX staining of thymic epithelial cells

Spleen

Spleen – Distinct nuclear NFIX staining of some stromal and endotheliel cells. Lymphocytes are NFIX negative

Tonsil

Tonsil – Distinct nuclear NFIX staining of a subset of squamous epithelial cells. Decreasing staining intensity from basal to the superficial cell layers. Some stromal cells and endothelial cells are strongly positive. Lymphocytes are largely negativ