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Pancreas with strong cytoplasmic C-peptide staining of most islet cells

Staining Pattern in Normal Tissues

Manual protocol

Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply HMV363 at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

BrainCerebrum, grey Negative
Cerebrum, white Negative
Cerebellum, cortex Negative
Cerebellum, white Negative
Ganglion Negative
Ependyma Negative
Eye, retina Negative
Endocrine TissuesThyroid Negative
Parathyroid gland Negative
Adrenal gland Negative
Pituitary gland, anterior lobe Negative
Pituitary gland, posterior lobe Negative
Respiratory systemLung bronchi Negative
Lung, bronchial glands Negative
Nose, paranasal sinus Negative
Lung, parenchyma Negative
Proximal digestive tractLip Negative
Oral cavity Negative
Tonsil, surface Negative
Esophagus, mucosa Negative
Lip, small salivary gland Negative
Sublingual gland Negative
Parotid gland Negative
Submandibullary gland Negative
Gastronintestinal tractStomach, antrum Negative
Stomach, fundus and corpus Negative
Small intestine, duodenum Negative
Duodenum, Brunner gland Negative
Small intestine, ileum Negative
Appendix Negative
Colon descendens Negative
Rectum Negative
Anal canal, transition epithelium Negative
Liver, Gallbladder, PancreasLiver Negative
Gallbladder Negative
Pancreas Strong cytoplasmic C-peptide immunostaining of the majority of islet cells. A faint staining of acinar cells surrounding pancreatic islets can be seen because of „contamination artifacts“.
Kidney, urinary bladderKidney, cortex Negative
Kidney, medulla Negative
Urinary bladder, urothelium Negative
Kidney pelvis, mucosa Negative
Male tissuesProstate Negative
Seminal vesicle Negative
Epididymis caput Negative
Epididymis cauda Negative
Testis Negative
Female TissuesBreast, glands Negative
Ectocervix Negative
Endocervix Negative
Endometrium, proliferation Negative
Endometrium, secretion Negative
Uterus, myometrium Negative
Fallopian tube Negative
Ovary, stroma Negative
Ovary, follicular cyst Negative
Ovary, corpus luteum Negative
Amnion Negative
Chorion Negative
Amnion/Chorion Negative
Placenta, early, decidua Negative
Placenta, first trimenon Negative
Placenta, mature Negative
Muscle, connective & soft tissueAorta, intima Negative
Skeletal muscle Negative
Aorta, media Negative
Skeletal muscle, tongue Negative
Heart, left ventricle Negative
Kidney pelvis, muscular wall Negative
Urinary bladder, muscular wall Negative
Esophagus, muscular wall Negative
Stomach, muscular wall Negative
Ileum, muscular wall Negative
Appendix, muscular wall Negative
Colon descendens, muscular wall Negative
Penis, glans, corpus spongiosum Negative
Fat, white Negative
SkinSkin, surface Negative
Skin (hairs, sebaceous glands) Negative
Anal canal, skin Negative
Scrotum Negative
Bone Marrow & lymphoid tissuesBone marrow Negative
Thymus Negative
Spleen Negative
Lymph node Negative
Tonsil, deep Negative

C-Peptide

(HMV363)

C-Peptide is a surrogate marker for insulin producing cells.

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From €295.00
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C-Peptide (HMV363)—
€295.00

Details

Type
Recombinant Rabbit monoclonal / IgG
Clone
HMV363
Reactivity
Human

More product details

Biology behind

C-peptide (connecting peptide) is a part of the proinsulin protein which is coded by the insulin gene at 11p15.5 and produced exclusively by the beta cells of the pancreatic islets. C-peptide is formed when the proinsulin is split into insulin and C-peptide. At that time equimolar quantities of insulin and C-peptide are released to the blood. C-peptide binds to the surface of several cell types (neuronal, endothelial, fibroblast and renal tubular) and can activate specific pathways. The clinical significance of C-peptide lies in its serological measurement as a parameter for endogenous insulin production (not influenced by exogenous insulin). 

Protocol Recommendations

Potential Research Applications

Evidence For Specificity In I H C